Suitable reference genes for relative quantification of miRNA expression in prostate cancer
نویسندگان
چکیده
منابع مشابه
Validation of suitable endogenous control genes for expression studies of miRNA in prostate cancer tissues.
When performing quantitative polymerase chain reaction analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used...
متن کاملValidation of Suitable Endogenous Control Genes for Expression Studies of Mirna in Prostate Cancer Tissues Short Title: Mirna Control Genes Prostate Cancer
When performing qPCR analysis, there is a need for correction of technical variation between experiments. This correction is most commonly performed by using endogenous control genes, which are stably expressed across samples, as reference genes for normal expression in a specific tissue. In microRNA (miRNA) studies, two types of control genes are commonly used and these are small nuclear RNAs ...
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Circulating cell-free microRNAs (miRNAs) are potential biomarkers of cancer. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is widely used in miRNA expression studies. The aim of this study was to identify suitable reference genes for RT-qPCR analyses of miRNA expression levels in pleural effusion. The expression levels of candidate reference miRNAs were investigated in ...
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BACKGROUND Relative quantification is a commonly used method for assessing gene expression, however its accuracy and reliability is dependent upon the choice of an optimal endogenous control gene, and such choice cannot be made a priori. There is limited information available on suitable reference genes to be used for studies involving human epicardial adipose tissue. The objective of the curre...
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ژورنال
عنوان ژورنال: Experimental and Molecular Medicine
سال: 2010
ISSN: 1226-3613
DOI: 10.3858/emm.2010.42.11.076